Formation of Cyclobutane Thymine Dimers from UVA Photosensitization of Pyridopsoralen Monoadducted DNA |
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| Authors: | Lidia Andreu Guillo,Bé né dicte Beylot,Paul Vigny,Annick Spassky |
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| Affiliation: | Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo Brazil;Laboratoire de Chimie et Biochimie Pharmacologique et Toxicologique, UniversitéRenéDescartes, Paris, France;Centre de Biophysique Moléculaire, Orléans, France |
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| Abstract: | The present report provides evidence that thymine dimerization can be UVA photosensitized at a tetranucleotide, 5′-TATT-3′, by a 7-methyl-pyrido(3, 4-c)psoralen monoadduct in DNA. The efficiency of the photoprocess depends on the tetranucleotide flanking sequences. These results demonstrate that one DNA lesion can originate the contiguous formation of a second type of lesion and emphasize the sequence-specific response to interaction of drugs with DNA. Results are related to the sensitivity of DNA to 1, 10-phenanthroline-cuprous ion complex nucleolytic activity and discussed in terms of the major role of local deformability of DNA in interaction with ligands. |
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