The novel use of gas chromatography-ion mobility-time of flight mass spectrometry with secondary electrospray ionization for complex mixture analysis |
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Authors: | Christina L Crawford Stephan Graf Marc Gonin Katrin Fuhrer Xing Zhang Jr" target="_blank">Herbert H HillJr |
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Institution: | (1) Department of Chemistry, Washington State University, PO Box 644630, Pullman, WA 99164, USA;(2) Tofwerk AG, Uttigenstrasse 22, CH-3600 Thun, Switzerland; |
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Abstract: | Increasing the dimensionality of an analysis enables more detailed and comprehensive investigations of complex mixtures. One
dimensional separation techniques like gas chromatography (GC) and ion mobility spectrometry (IMS) provide limited chemical
information about complex mixtures. The combination of GC, ion mobility spectrometry, and time-of-flight mass spectrometry
(GC-IM-TOFMS) provides three-dimensional separation of complex mixtures. In this work, a hybrid GC-IM-TOFMS with a secondary
electrospray ionization (SESI) source provided four types of analytical information: GC retention time, ion mobility drift
time, mass-to-charge ratios, and ion intensity. The use of secondary electrospray ionization enables efficient and soft ionization
of gaseous sample vapors at atmospheric pressure. Several complex mixtures, including lavender and peppermint essential oils,
were analyzed by GC-SESI-IM-TOFMS. The resulting 3D data from these mixtures, each containing greater than 50 components,
were plotted as 3D projections. In particular, post-processed data plotted in three dimensions showed that many mass selected
GC peaks were resolved into different ion mobility peaks. This technique shows clear promise for further in-depth analyses
of complex chemical and biological mixtures. |
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