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Single-run analysis of retinal isomers, retinol and photooxidation products by high-performance liquid chromatography |
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| Authors: | Cyrille Ceugniet L&#x;titia Lepetit Nancy Lauth De Viguerie Henry Jammes Nicole Peyrot Monique Rivire |
| Institution: | a Laboratoire IMRCP, UMR 5623, Université Paul Sabatier, 118 Route de Narbonne, F-31062 Toulouse Cedex 04, France b Laboratoire Pierre Fabre Dermo-Cosmétique, Allée Camille Soula, Vigoulet Auzil, 31322 Castanet Tolosan, France |
| Abstract: | A high-performance liquid chromatographic (HPLC) procedure was developed to allow the rapid separation, in a single run, of a mixture of the main retinal isomers (all-trans, 13-cis, 9-cis), all-trans-retinol, and of the two major photooxygenated photoproducts (5,8-peroxyretinal and 5,6-epoxyretinal). The mixture was separated by HPLC on an octadecyl (ODS) column with 16% (v/v) diethyl ether in hexane as mobile phase and anthracene as the internal standard. A commercial type cosmetic formulation containing 0.05% all-trans-retinal was analyzed successfully for this analyte. |
| Keywords: | Retinal Retinol Retinoids |
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