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Self-assembled luminescent CdSe-ZnS quantum dot bioconjugates prepared using engineered poly-histidine terminated proteins |
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| Authors: | Ellen R. Goldman Igor L. Medintz George P. Anderson Brent L. Iverson George Georgiou Hedi Mattoussi |
| Affiliation: | a Center for Bio/Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA b Department of Chemical Engineering, University of Texas, Austin, TX 78712, USA c Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA d Department of Chemistry and Biochemistry, University of Texas, Austin, TX 78712, USA e Division of Optical Sciences, Code 5611, US Naval Research Laboratory, Washington, DC 20375, USA |
| Abstract: | We report a simple and versatile approach for the conjugation of luminescent CdSe-ZnS core-shell quantum dots (QDs) to proteins through coordination of engineered C-terminal oligohistidine sequences. Several histidine tail containing proteins were self-assembled onto the QD surface using this method. A recombinant antibody specific for the high explosive 2,4,6-trinitrotoluene (TNT) was conjugated to QDs through a carboxy terminal histidine tail and the bioconjugate used to detect TNT by competitive immunoassay. TNT was detected over the range of 10 μg/ml down to 41 ng/ml using the scFv conjugated to QDs. These results open up the possibility to conjugate luminescent QDs to a whole range of proteins to form QD bioconjugates that can be effectively used in bio-oriented applications, such as sensing, imaging, immunoassay and other diagnostics. |
| Keywords: | Quantum dot Histidine tag Affinity tag Bioconjugation Single chain antibody scFv TNT Immunoassay |
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