W/0乳化法制备明胶/β-磷酸三钙多孔复合微球及其物化性能表征

采用乳液、离子缔合法制备得到明胶（Gel）/β-纳米磷酸三钙(β-TCP)复合多孔微球，其尺寸可通过控制反应的搅拌速度进行调节。 SEM和光学显微镜观察表明，明胶/β-TCP复合微球尺寸在20～40 μm之间，被包敷的磷酸三钙为200 nm左右，微球内部呈多孔结构。 当m（磷酸三钙）∶m（明胶）＞0.4∶1时，有大量花瓣状晶体附着于复合微球的表面，是磷酸三钙溶解和明胶分子诱导重结晶所致。 XRD与IR图谱表明，磷酸三钙纳米粒子与明胶之间存在化学键合，明胶/β-纳米磷酸三钙复合微球的微观结构与自然骨相似。 DSC-TGA结果显示，90%的TCP在乳化过程中与明胶复合。 本文所制备的复合微球，为添加各种药物和促骨生长因子并实现缓释提供了优良的载体。

The preparation and characterization of gelatin/β-tricalcium phosphate porous composite spheres by W/O emulsion process

Through W/O emulsion process and ion association method, Gelatin/β-tricalcium phosphate porous composite spheres were fabricated. The size of the composite spheres was controlled by adjusting the rate of stirring. From the SEM image, can it be seen that the size of the composite spheres was arranged from 5um to 30um, and the TCP embedded is about 200nm. When the amount of the TCP added to the composite is up to 0.4:1, a large quantities of petal-like crystal were formed on the surface of the composite spheres. It was assumed that shaped crystal was formed by TCP dissolved in the reactive solution and inductive recrystallization by gelatin molecule. IR and XRD spectrum showed these composite spheres containing the similar structure with the natural bone and the chemical bond existed between the gelatin and TCP. DSC-TGA image illustrated the 90% TCP was embedded inside the gelatin spheres through the emulsion process and strong interaction appeared between them.