Using light to bioactivate surfaces: A new way of creating oriented, active immunobiosensors |
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Authors: | Meg Duroux Leonid Gurevich Esben Skovsen Steffen B Petersen |
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Institution: | a Nanobiotechnology Group, Department of Physics and Nanotechnology, Aalborg University, Skjernvej 4A, DK-9220 Aalborg, Denmark b Stem Cell Research, Department of Health Science and Technology, Aalborg University, Fredrik Bajers Vej 3B, DK-9220 Aalborg, Denmark c Institute for Lasers, Photonics and Biophotonics, University at Buffalo, 428 Natural Science Complex, Buffalo, NY 14260-3000, USA |
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Abstract: | Ultraviolet light can be used to immobilize biomolecules onto thiol reactive surfaces in order to, e.g., make biosensors. The mechanism involves light-induced formation of free, reactive thiol groups in disulphide containing molecules. This technology allows for the creation of arrays of biomolecules with a high degree of reproducibility, circumventing the need for often expensive nano/micro-dispensing technologies. The ultimate size of the immobilized spots is defined by the focal area of the UV beam. Light-induced immobilization has the added benefit that the immobilized molecules will be spatially oriented and covalently bound to the surface. In this paper, we demonstrate the utility of a sensor array created with the new sensor technology when integrated into a microfluidic system. Protein arrays made using light-induced immobilization showed successful antigen/antibody binding in a flow cell allowing the visualisation of real time binding and enzyme activity. This new technology is ideal for the creation of protein/DNA microarrays, can replace present micro-dispensing arraying technologies and is ideal as a molecular imprinting technology. |
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Keywords: | Protein arrays UV light Biosensors Microfluidics Fluorescence microscopy Light assisted protein immobilization Fab fragments |
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