分子印迹聚合物的制备及孔结构研究

以N 叔丁氧羰酰 L 色氨酸和N 叔丁氧羰酰 L 酪氨酸为印迹分子 ,分别采用光引发聚合和热引发聚合制备了分子印迹聚合物 ,并对聚合物的手性识别能力进行了色谱评价 .结果表明 ,制备的分子印迹聚合物对印迹分子具有特异性的吸附作用 ,光引发聚合的N 叔丁氧羰酰 L 色氨酸的印迹聚合物对印迹分子的选择性因子达到 2 .318,热引发聚合的N 叔丁氧羰酰 L 酪氨酸对印迹分子的选择性因子为 1 373 进一步研究了分子印迹聚合物的孔结构 ,发现光引发聚合的分子印迹聚合物与空白聚合物的孔结构差别比热引发聚合的分子印迹聚合物与空白聚合物的差别更为明显 .对印迹分子洗脱前后的印迹聚合物的孔结构研究进一步表明 ,印迹分子存在于不同类型的孔中 .

PREPARATION OF MOLECULARLY IMPRINTED POLYMERS AND STUDIES ON THEIR PORE-STRUCTURE

In the presence of template molecules of N Boc L Tryptophan (Trp) and N Boc L Tyramine (Tyr),two kinds of molecularly imprinted polymers (MIPs) were synthesized by photo induced and thermal induced polymerization,respectively. High pressure liquid chromatography analyses using MIPs as the stationary phase showed that both MIPs could specifically recognize the corresponding imprinted molecules from the racemic mixtures of amino acid derivatives.The separation factors of N Boc L Trp and N Boc L Tyr on the corresponding MIPs were 2 318 and 1 373,respectively. The surface structure observation of MIPs by Scanning Electronic Microscopy showed that both MIPs and blank polymers polymerized by photo and thermal inducement were porous granules with different kinds of pores. From pore structure analyses, some differences between MIPs and blank polymers in pore structures were found,and the difference in pore diameter between MIPs and blank polymers by photo induced polymerization was more obvious than that of the thermal induced polymers.When the imprinted molecules were removed from MIPs by elution,the structures of pores of various sizes in the MIPs were all markedly changed,showing that the imprinted molecules could be adsorbed by MIPs in any kinds of pores. All these results indicated that the pore structure of MIPs played very important roles in the process of molecular recognition of the imprinted molecules.