高效液相色谱法测定食品中氟啶脲的残留量

建立了高效液相色谱测定食品中氟啶脲残留量的方法.样品中的氟啶脲经正己烷或乙腈提取,弗罗里硅土净化后,以乙腈-水( 85:15,v/v)混合溶液为流动相,经C18色谱柱分离,紫外检测器(260 nm)测定.结果表明:氟啶脲在0 05 ～2.0 mg/L范围内线性良好(相关系数为0 999 8),定量限(以信噪比为10计)...

Determination of chlorfluazuron residue in foods by high performance liquid chromatography

A method for the determination of chlorfluazuron residue in foods by high performance liquid chromatography (HPLC) was developed. The samples were extracted with hexane or acetonitrile. After cleaned up with Florisil-solid phase extraction (SPE), the samples were analyzed by the separation on a C18 chromatographic column with an acetonitrile and water (85 : 15, v/v) mixed solution as the mobile phase and the determination with an ultraviolet detector at 260 nm. The linear range was 0.05 - 2.0 mg/L, the correlation coefficient was 0.999 8 and the limit of quantification (S/N = 10) was 0.05 mg/kg. The recoveries were 82.1% - 102.5% with the relative standard deviations (n = 10) of 3.00% - 6.25% at the three different spiked levels of 0.05, 0.1 and 1 mg/kg. The method is easy, fast, accurate and consuming less sample and organic solvents. It can be applied in the determination of chlorfluazuron in foods.