| 纤维素键合型手性整体柱的制备及其在快速手性分离中的应用 |
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| 引用本文： | Wang J,Wang X,Li J,Lü H,Lin X,Xie Z,Zhang Q.纤维素键合型手性整体柱的制备及其在快速手性分离中的应用[J].色谱,2011,29(12):1222-1229. |
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| 作者姓名： | Wang J Wang X Li J Lü H Lin X Xie Z Zhang Q |
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| 作者单位： | 1. 福州大学生物和医药技术研究院, 福建 福州 350002; 2. 福州大学食品安全与环境监测技术研究所, 福建 福州 350108; 3. 福州大学材料科学与工程学院, 福建 福州 350108 |
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| 基金项目： | 福建省科技厅重大专项(2010NZ0001-1)和福州大学科技发展基金项目(2010-XQ-17) |
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| 摘 要： | 采用N-丙烯酰琥珀酰亚胺(NAS)为基质单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,原位聚合制备聚(NAS-co-EDMA)毛细管整体柱,并通过化学键合法将自合成的纤维素-三(4-甲基苯甲酸酯)(CTMB)共价键合到整体柱上,制备用于快速手性分离的纤维素键合型手性整体柱.优化了整体柱制备和衍生化条件；通过对固定相红...
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| 关 键 词： | 纤维素-三(4-甲基苯甲酸酯) 共价键合 手性毛细管整体柱 毛细管电色谱 快速手性分离 |
| 收稿时间： | 2011-07-06 |
Reparation of chiral monolithic column with covalently bonded cellulose and their application to rapid enantioseparation |
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| Wang Jiabin,Wang Xiao,Li Jianhua,Lü Haixia,Lin Xucong,Xie Zenghong,Zhang Qiqing.Reparation of chiral monolithic column with covalently bonded cellulose and their application to rapid enantioseparation[J].Chinese Journal of Chromatography,2011,29(12):1222-1229. |
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| Authors: | Wang Jiabin Wang Xiao Li Jianhua Lü Haixia Lin Xucong Xie Zenghong Zhang Qiqing |
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| Institution: | 1. Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China; 2. Institute of Food Safety and Environmental Monitoring, Fuzhou University, Fuzhou 350108, China; 3. College of Materials Science and Engineering, Fuzhou University, Fuzhou 350108, China |
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| Abstract: | A chiral monolithic capillary column for rapid enantioseparation was prepared by covalently bonding of cellulose tris(4-methylbenzoate) (CTMB) on N-acryloxysuccinimide-based monolith. The preparation and derivatization conditions of the monolithic column were optimized. The successful grafting of CTMB was confirmed on the characterizations of the infrared spectrum and the cathodic electroosmotic flow (EOF). The effects of acetic acid concentration and methanol content on the enantioseparation were studied. The solvent resistance, reproducibility and stability of the monolithic column have also been investigated. The rapid enantioseparation of the five solutes (phenylalanine, tyrosine, tryptophan, propranolol and phenylethanol) with resolution (R(s)) values up to 1.31 was achieved within 1.2 min on the prepared chiral capillary monolithic column by capillary electrochromatography. |
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| Keywords: | cellulose tris(4-methylbenzoate) covalent bonding chiral monolithic capillary column capillary electrochromatography (CEC) rapid enantioseparation |
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