Determination of total ammonium-nitrogen and free ammonia in a fermentation medium by sequential injection analysis |
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Authors: | Ingrid Lukkari Jaromir Ruzicka Gary D. Christian |
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Affiliation: | (1) Department of Analytical Chemistry, University of Umeå, S-901 87 Umeå, Sweden;(2) Department of Chemistry BG-10, University of Washington, 98195 Seattle, WA, USA |
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Abstract: | Summary Automated methods for the determination of ammonium and ammonia are reviewed, and techniques based on gas diffusion using a semi-permeable membrane were selected for the determination of both total ammonium-nitrogen and free ammonia in fermentation samples. A simple and robust instrument based on sequential injection analysis (SIA) consisting of a piston pump and two selector valves was used. Two different methods of ammonia detection, the Berthelot method and detection using pH-indicators, have been evaluated and compared. The indicator method showed higher reproducibility and the range of determination could easily be adjusted to match the concentrations of the samples. The range of determination could be adjusted from 0.05 mmol/l to 350 mmol/l, depending on choice of acceptor solution. This method has been evaluated with fermentation medium samples and tested on-line in a yeast fermentation process.Dedicated to Professor Dr. Wilhelm Fresenius on the occasion of his 80th birthday |
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