| Institution: | 1. M. A. Kazi Institute of Chemistry, University of Sindh, Jamshoro, Pakistan;2. Department of Chemistry, Quaid-I-Azam University, Islamabad, Pakistan;3. Department of Biology, College of Science, University of Bahrain, Sakhir, Bahrain;4. Department of Chemistry, University of Gujrat, Gujrat, Pakistan
Department of Chemistry, University of Agriculture, Faisalabad, Pakistan;5. Core Research Facilities, King Fahd University of Petroleum & Minerals, Dhahran, Saudi Arabia;6. Institute of Molecular Biology and Biotechnology/(IMBB), The University of Lahore, Lahore, Pakistan;7. Department of Biological Sciences, College of Natural Sciences, Kongju National University, Chungnam, South Korea |
| Abstract: | The porcine pancreatic elastase inhibition and free-radical scavenging play a crucial role in age progression. All the series of 10 newly synthesized benzimidazole thioureas ( 4a-j ) were assessed for elastase inhibition and radical scavenging activity to identify the suitable anti-aging ingredient for cosmetics products. The compounds 4e , 4f , 4g , and 4h showed inhibition better than the standard, while compound 4f showed the most significant elastase inhibition with the IC50 value of 1.318 ± 0.025 μM compared with oleanic acid IC50 13.451 ± 0.014 used ±1.989 and 41.563 ± 0.824, respectively, as standard. Molecular docking studies were performed and the compound 4f showed binding energy of 7.2 kcal/mol. Kinetics studies revealed inhibition of the pancreatic elastase in a competitive manner. The relative binding energy and structure activity relationship (SAR) identified compound 4f as an effective inhibitor of porcine pancreatic elastase. Compounds 4e and 4i showed remarkable free-radical scavenging activity with SC50 values of 26.421. |
