| 高速逆流色谱分离纯化EGCG3"Me的研究 |
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| 引用本文： | 罗正飞,金孝芳,龚正礼,梁名志,王立波,陈继伟.高速逆流色谱分离纯化EGCG3"Me的研究[J].分析测试学报,2011,30(7):813-816. |
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| 作者姓名： | 罗正飞 金孝芳 龚正礼 梁名志 王立波 陈继伟 |
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| 作者单位： | 1.西南大学食品科学学院，2.云南省农业科学院茶叶研究所，3.湖北省农业科学院果树茶叶研究所 |
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| 基金项目： | 重庆市科委项目资助（GSTC.2007AC1044） |
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| 摘 要： | 首次采用高速逆流色谱法对经自制聚酰胺初步分离的表没食子儿茶素-3-(3″-O-甲基)没食子酸酯( EGCG3"Me)样品中的EGCG3"Me单体进行分离纯化.结果表明,选择水-甲醇-乙酸乙酯-正己烷(体积比5:2:9:1)为高速逆流色谱分离的两相溶剂系统,上相为固定相,下相为流动相,在主机转速700 r／min、流速2...
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| 关 键 词： | EGCG3"Me 高速逆流色谱 分离纯化 高效液相色谱 |
Simultaneous Determination of Dopamine,Uric Acid and Ascorbic Acid in Urine Sample by Differential Pulse Voltammetry and Artificial Neural Networks |
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| LUO Zheng-fei,JIN Xiao-fang,GONG Zheng-li,LIANG Ming-zhi,WANG Li-bo,CHEN Ji-wei.Simultaneous Determination of Dopamine,Uric Acid and Ascorbic Acid in Urine Sample by Differential Pulse Voltammetry and Artificial Neural Networks[J].Journal of Instrumental Analysis,2011,30(7):813-816. |
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| Authors: | LUO Zheng-fei JIN Xiao-fang GONG Zheng-li LIANG Ming-zhi WANG Li-bo CHEN Ji-wei |
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| Institution: | LUO Zheng-fei1,2,JIN Xiao-fang1,3,GONG Zheng-li1,LIANG Ming-zhi2,WANG Li-bo2,CHEN Ji-wei2(1.College of Food Science,Southwest University,Chongqing 400716,China,2.Tea Research Institute,Yunnan Academy of Agricultural Sciences,Menghai 666201,3.Fruit and Tea Research Institute,Hubei Academy of Agricultural Sciences,Wuhan 430209,China) |
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| Abstract: | A method for the isolation and purification of(-)-epigallocatechin-3-(3″-O-methyl)gallate(EGCG3″Me) from crude extract sample was firstly developed by high speed countercurrent chromatography(HSCCC).The results showed that HSCCC had a two-phase solvent system composing of water-methanol-ethyl acetate-hexane(5 ∶2 ∶9 ∶1,by volume) on a preparative scale.The upper phase was used as the stationary phase,and the lower phase was used as the mobile phase.The revolution speed was 700 r/min,the flow rate was 2.0 mL/min and the detection wavelength was set at 254 nm.Under the optimized conditions,EGCG3″Me can be well isolated.The purity of EGCG3″Me detected by high performance liquid chromatography was more than 90%,and the yield of EGCG3″Me by HSCCC was 76.53%. |
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| Keywords: | EGCG3″Me high speed countercurrent chromatography isolation and purification high performance liquid chromatography |
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