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Determination of phenol and catechol concentrations with oxygen probes coated with immobilized enzymes or immobilized cells |
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| Authors: | Halina Y Neujahr |
| Institution: | (1) Department of Biochemistry and Biotechnology, The Royal Institute of Technology, S-100 44 Stockholm, Sweden |
| Abstract: | The enzyme phenol 2-hydroxylase was immobilized on Sepharose and used in conjunction with an O2 electrode for quantitating phenol. Similarly, catechol 1,2-oxygenase was used for quantitating catechol. A third probe was prepared by immobilization ofTrichosporon cutaneum cells rather than purified phenol 2-hydroxylase for phenol quantitation. The whole cell system gave results comparable to the immobilized enzyme system. |
| Keywords: | Phenol determinations with immobilized enzymes and cells catechol determination with immobilized enzymes and cells immobilized enzymes and cells determination of phenols and catechols with enzymes immobilized in determinations of phenols and catechols cells immobilized in determination of phenols and catechols Trichosporon cutaneum |
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