High‐Resolution Crystal Structure of a Silver(I)–RNA Hybrid Duplex Containing Watson–Crick‐like CSilver(I)C Metallo‐Base Pairs |
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| Authors: | Dr Jiro Kondo Yoshinari Tada Dr Takenori Dairaku Dr Hisao Saneyoshi Dr Itaru Okamoto Prof Yoshiyuki Tanaka Prof Akira Ono |
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| Institution: | 1. Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7‐1 Kioi‐cho, Chiyoda‐ku, Tokyo 102‐8554 (Japan);2. Graduate School of Science and Technology, Sophia University, 7‐1 Kioi‐cho, Chiyoda‐ku, Tokyo 102‐8554 (Japan);3. Laboratory of Molecular Transformation, Graduate School of Pharmaceutical Sciences, Tohoku University, 6‐3 Aza‐Aoba, Aramaki, Aoba‐ku, Sendai 980‐8578 (Japan);4. Department of Material and Life Chemistry, Faculty of Engineering, Kanagawa University, 3‐27‐1 Rokkakubashi, Kanagawa‐ku, Yokohama 221‐8686 (Japan);5. Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro‐cho, 770‐8514 Tokushima (Japan) |
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| Abstract: | Metallo‐base pairs have been extensively studied for applications in nucleic acid‐based nanodevices and genetic code expansion. Metallo‐base pairs composed of natural nucleobases are attractive because nanodevices containing natural metallo‐base pairs can be easily prepared from commercially available sources. Previously, we have reported a crystal structure of a DNA duplex containing T? HgII? T base pairs. Herein, we have determined a high‐resolution crystal structure of the second natural metallo‐base pair between pyrimidine bases C? AgI? C formed in an RNA duplex. One AgI occupies the center between two cytosines and forms a C? AgI? C base pair through N3? AgI? N3 linear coordination. The C? AgI? C base pair formation does not disturb the standard A‐form conformation of RNA. Since the C? AgI? C base pair is structurally similar to the canonical Watson–Crick base pairs, it can be a useful building block for structure‐based design and fabrication of nucleic acid‐based nanodevices. |
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| Keywords: | crystal structure metallo‐base pairs RNA silver X‐ray analysis |
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