Characterization of a Water-Dispersed Biodegradable Polyurethane-Silk Composite Sponge Using 13C Solid-State Nuclear Magnetic Resonance as Coating Material for Silk Vascular Grafts with Small Diameters |
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Authors: | Takashi Tanaka Yusuke Ibe Takaki Jono Ryo Tanaka Akira Naito Tetsuo Asakura |
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Institution: | 1.Department of Veterinary Science, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan; (T.T.); (R.T.);2.Polyurethane Research Laboratory, Tosoh Corporation, Mie 510-8540, Japan; (Y.I.); (T.J.);3.Department of Biotechnology, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan; |
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Abstract: | Recently, Bombyx mori silk fibroin (SF) has been shown to be a suitable material for vascular prostheses for small arteries. In this study, we developed a softer SF graft by coating water-dispersed biodegradable polyurethane (PU) based on polycaprolactone and an SF composite sponge on the knitted SF vascular graft. Three kinds of 13C solid-state nuclear magnetic resonance (NMR), namely carbon-13 (13C) cross-polarization/magic angle spinning (MAS), 13C dipolar decoupled MAS, and 13C refocused insensitive nuclei enhanced by polarization transfer (r-INEPT) NMR, were used to characterize the PU-SF coating sponge. Especially the 13C r-INEPT NMR spectrum of water-dispersed biodegradable PU showed that both main components of the non-crystalline domain of PU and amorphous domain of SF were highly mobile in the hydrated state. Then, the small-diameter SF artificial vascular grafts coated with this sponge were evaluated through implantation experiments with rats. The implanted PU-SF-coated SF grafts showed a high patency rate. It was confirmed that the inside of the SF grafts was covered with vascular endothelial cells 4 weeks after implantation. These results showed that the water-dispersed biodegradable PU-SF-coated SF graft created in this study could be a strong candidate for small-diameter artificial vascular graft. |
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Keywords: | silk fibroin small-diameter artificial vascular graft 13C solid-state nuclear magnetic resonance biodegradable polyurethane |
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