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Determination of catecholamines in human serum by micro high-performance liquid chromatography with micro precolumn and dual electrochemical detection
Authors:M Goto  G Zou  D Ishii
Abstract:
A dual electrochemical detector having two working electrodes (anode and cathode) in parallel--opposed configuration suitable for micro high-performance liquid chromatography was developed for the selective and sensitive detection of catecholamines on the basis of their electrochemical reversibility and catalytic amplification by recycling oxidation and re-reduction. The micro high-performance liquid chromatographic system with a micro alumina precolumn for enriching catecholamines and the dual electrochemical detector in parallel--opposed configuration was successfully utilized for the determination of catecholamines in healthy human serum injected directly after ultrafiltration.
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