Evaluation of a mixed immunosorbent for selective trace enrichment of phenylurea herbicides from plant material |
| |
Authors: | S Herrera A Martín-Esteban P Fernández D Stevenson and C Cámara |
| |
Institution: | (1) Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, E-28040 Madrid, Spain, ES;(2) Robens Analytical Chemistry Centre, School of Biological Science, University of Surrey, Guildford, Surrey GU2 5XH, United Kingdom, GB |
| |
Abstract: | A mixed immunosorbent, composed of anti-isoproturon and anti-chlortoluron antibodies immobilized on aldehyde-activated silica
was evaluated for the trace enrichment and clean-up of several phenylurea herbicides from potatoes, carrots and peas. As the
immunosorbent exhibited non-specific interactions with the matrix components, a previous clean-up step based on gel filtration
(Sephadex G-25 PD-10 column) or anion-exchange (Power 1 × 8 Dionex column) was necessary. The coupling of gel filtration and
immunosorbent with subsequent HPLC/UV determination allowed the determination of selected herbicides in potatoes in the 30
ng/g range with high precision, but for more complex samples (carrots and peas) the combination of anion-exchange resin and
immunosorbent was required. With 30 ng/g spiked samples recovery rates for isoproturon, chlortoluron, metobromuron, linuron
and chlorbromuron averaged 80 ± 20% (n = 5 in two consecutive days) for the whole procedure and the estimated detection limits ranged from 5 to 20 ng/g.
Received: 29 January 1998 / Revised: 4 May 1998 / Accepted: 30 May 1998 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|