High-sensitivity observation of dipolar exchange and NOEs between exchangeable protons in proteins by 3D solid-state NMR spectroscopy |
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| Authors: | Paulson Eric K Morcombe Corey R Gaponenko Vadim Dancheck Barbara Byrd R Andrew Zilm Kurt W |
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| Institution: | Department of Chemistry, Yale University, P.O. Box 208107, New Haven, CT 06520-8107, USA. |
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| Abstract: | A highly sensitive new 1H-detected 3D solid-state NMR method is described for characterizing 1H-1H spin exchange in nanocrystalline samples of 15N- and 2H-enriched protein. Long-range contacts are observed in human ubiquitin. The method is also used to show that numerous NOEs between backbone amides and crystal water protons can be observed. |
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