液质联用技术分析西洋参花化学成分及H9c2心肌细胞损伤保护作用

高温高压转化前后西洋参花醇提液经正己烷、氯仿、乙酸乙酯和正丁醇分级萃取后共得10种不同极性组分，通过H9c2心肌细胞损伤模型筛选最佳活性组分，并对其进行抗氧化活性的测定以及化学组成分析，利用液质联用技术检测其处理前后的化学成分。结果表明，提取的10种活性组分中，高温高压转化后西洋参花乙酸乙酯萃取层(PEE)能够有效保护H9c2心肌细胞损伤，显著提高细胞存活率并降低细胞ROS水平，与高温高压转化处理前西洋参花乙酸乙酯层(OEE)相较，二者总体皂苷含量相近且均具有较强的抗氧化活性。通过液质分析，初步确定了PEE中所含高温高压转化裂解生成的Rk₁与Rg₅更具良好的心肌细胞损伤保护作用。

Study on Extract Composition of American Ginseng Flower in Oxidative-Induced H9c2 Cardromyocytes by LC-MS

In this study， the American ginseng flower is pre-treated by high temperature and pressure and the ethanol extract of American ginseng flower is divided by n-hexane， chloroform， ethyl acetate and n-butanol consequently. A total of 10 different polar components are obtained. The best active fraction is screened by oxidative-induced H9c2 Cardromyocytes. The ability of free radical scavenging and chemical composition of the fractions are analyzed by LC-MS. The results show that the processed ethyl acetate extraction （PEE） of American ginseng flowers effectively protects oxidative-induced H9c2 Cardromyocytes， significantly improves cell viability and reduces cell ROS levels. Compared with the original ethyl acetate extraction （OEE）， the ginsenoside content of the two groups is similar and shows strong antioxidant capacity. Through LC-MS analysis， it is preliminarily determined that Rk₁ and Rg₅ generated by high temperature and pressure transformation in PEE. Therefore， processed American ginseng flower has better protective effect on oxidative-induced H9c2 Cardromyocytes than original.