1H NMR分析肝素钠中杂质多硫酸软骨素

对肝素钠、多硫酸软骨素标准品进行了¹H NMR测试并对谱图进行了全归属，指出: 肝素钠乙酰甲基特征峰在δ 2.04处，多硫酸软骨素的乙酰甲基特征峰在δ 2.15处. 同时考察了精细测试的实验条件，结果表明：当样品浓度为25 mg/0.6 mL、定标物TSP含量为0.020%（W/V）、样品旋转为12 Hz、处理参数（LB）为1.0、仪器频率为500 MHz时，能快速并准确的检测出肝素钠中的污染物(多硫酸软骨素).

Assessment of Oversulfated Chondroitin Sulfate in Heparin Sodium Solution with 1H NMR

The standard samples of heparin (HP) and oversulfated chondroitin sulfate (OSCS) were analyzed by ¹H NMR. The spectra were assigned. The results showed that the acetyl-methyl peaks of heparin and OSCS have different chemical shift, at δ2.04 and δ 2.15 respectively. The experimental conditions for quantitative analysis were studied. It was shown that, OSCS content in HP solution can be accurately measured under the following conditions: 25 mg sample in 0.6 mL D₂O, 0.020%（W/V）TSP, spinning rate 12 Hz, line broadening for EM window functions 1.0, and spectrometer frequency of 500 MHz.