杨树嫩茎质外体微透析液中K+,Na+,Ca2+的分析

采用微透析法获取3种杨树嫩茎质外体汁液的灌流液,流速1μL/min,探针截留分子量为30 kDa,无须处理直接通过原子吸收光谱(石墨炉法)测定样品中Na+,K+,Ca2+浓度,通过建立实时、无损检测离子浓度变化的实验技术,进行杨树生理生化的研究。此方法加标回收率在95.8%~103.1%之间。研究表明,吴屯杨、小胡杨和小美旱的Na+含量分别为1034~1156μg/L,1493~1611μg/L和1586~1703μg/L;K+含量分别为1012~1237μg/L,941~964μg/L和1095~1201μg/L;Ca2+含量分别为4976~5237μg/L,4786~5042μg/L和5893~6142μg/L,本方法为在植物应激反应中通过离子浓度变化来适应外界环境的研究提供可靠数据。

Determination of K+, Na+and Ca2+in Miorodialysis of Apoplast of Poplar Stems by Atomic Absorption Spectrometry with Microdialysis Method

The dialysis for three species of populus stems apoplast was carried out with a microdialysis system, and the concentration of Na⁺, K⁺ and Ca²⁺ in the dialysate was directly measured by the graphite furnace atomic absorption spectrometry (GF-AAS). The flow rate of perfusate was 1 μL min^?1 and the cut-off molecular weight of probe was about 30 kDa. The test technique was applied to the research of poplar biochemical physiology due to its perfect characteristic of real-time and non-destructive detection. The recovery of the method was 95.8%–103.1%. The content of Na⁺ in Populus wutunensis, Populus simonii × P.euphratica” × P.sp and Populus simonii × (Populus pyramidalis + Salix matsudana) were 1034–1156 μg L^?1, 1493–1611 μg L^?1 and 1586–1703 μg L^?1, respectively, K⁺ was 1012–1237 μg L^?1, 941–964 μg L^?1 and 1095–1201 μg L^?1, and Ca²⁺ was 4976–5237 μg L^?1, 4786–5042 μg L^?1 and 5893–6142 μg L^?1. This method could provide reliable data for the investigation of the change of ion concentration of plant to all kinds of external environment stress.