| 钌(II)配合物与酵母tRNA相互作用的等温滴定量热研究 |
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| 引用本文： | 徐宏,刘剑洪,刘志刚,梁毅,张鹏,杜芬,周兵瑞,计亮年.钌(II)配合物与酵母tRNA相互作用的等温滴定量热研究[J].中国化学,2005,23(6):659-661. |
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| 作者姓名： | 徐宏 刘剑洪 刘志刚 梁毅 张鹏 杜芬 周兵瑞 计亮年 |
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| 作者单位： | [1]DepartmentofChemistryandBiology,NormalCollege,ShenzhenUniversity,Shenzhen,Guangdong518060,China [2]CollegeofLifeSciences,WuhanUniversity,Wuhan,Hubei430072,China [5]StateKeyLaboratoryofCoordinationChemistry,NanjingUniversity,Nanjing,Jiangsu210093,China／／SchoolofChemistryandChemicalEngineering,ZhongshanUniversity,Guangzhou,Guangdong510275,China |
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| 摘 要： | The interaction of metal complex with RNA has been studied by isothermal titration calorimetry (ITC) for the first time. ITC experiments show that complex Ru(phen)2MPIP]^2 {phen= 1,10-phenanthroline, MPP-2-(4-methylphenyl)imidazo4,5-f]-1, 10-phenanthroline} interacts with yeast tRNA in terms of a model for a singleset of identical sites through intercalation, which is consistent with our previous observation obtained from spectroscopic methods, and this binding process was driven by a moderately favorable enthalpy decrease in combination with a moderately favorable entropy increase, suggesting that ITC is an effective method for deep studying the interactions of metal complexes with RNA.
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| 关 键 词： | 钌化合物 酵母 RNA 催化作用 化学结构 ITC技术 等温滴定热量测定法 |
| 收稿时间： | 2004-11-17 |
| 修稿时间： | 2005-3-18 |
Interaction of Ru(II) Complex with Yeast tRNA Studied by Isothermal Titration Calorimetry |
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| Xu Hong,Liu Jian‐Hong,Liu Zhi‐Gang,Liang Yi,Zhang Peng,Du Fen,Zhou Bing‐Rui,Ji Liang‐Nian.Interaction of Ru(II) Complex with Yeast tRNA Studied by Isothermal Titration Calorimetry[J].Chinese Journal of Chemistry,2005,23(6):659-661. |
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| Authors: | Xu Hong Liu Jian‐Hong Liu Zhi‐Gang Liang Yi Zhang Peng Du Fen Zhou Bing‐Rui Ji Liang‐Nian |
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| Abstract: | The interaction of metal complex with RNA has been studied by isothermal titration calorimetry (ITC) for the first time. ITC experiments show that complex Ru(phen)2MPIP]2+ {phen=1,10‐phenanthroline, MPIP=2‐(4‐methylphenyl)imidazo4,5‐f]‐1,10‐phenanthroline} interacts with yeast tRNA in terms of a model for a single set of identical sites through intercalation, which is consistent with our previous observation obtained from spectroscopic methods, and this binding process was driven by a moderately favorable enthalpy decrease in combination with a moderately favorable entropy increase, suggesting that ITC is an effective method for deep studying the interactions of metal complexes with RNA. |
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| Keywords: | ruthenium(II) complex yeast tRNA ITC interaction |
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