NAIMA as a solution for future GMO diagnostics challenges |
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Authors: | David Dobnik Dany Morisset Kristina Gruden |
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Institution: | (1) Department of Biotechnology and Systems Biology, National Institute of Biology, Vecna pot 111, Ljubljana, 1000, Slovenia; |
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Abstract: | In the field of genetically modified organism (GMO) diagnostics, real-time PCR has been the method of choice for target detection
and quantification in most laboratories. Despite its numerous advantages, however, the lack of a true multiplexing option
may render real-time PCR less practical in the face of future GMO detection challenges such as the multiplicity and increasing
complexity of new transgenic events, as well as the repeated occurrence of unauthorized GMOs on the market. In this context,
we recently reported the development of a novel multiplex quantitative DNA-based target amplification method, named NASBA
implemented microarray analysis (NAIMA), which is suitable for sensitive, specific and quantitative detection of GMOs on a
microarray. In this article, the performance of NAIMA is compared with that of real-time PCR, the focus being their performances
in view of the upcoming challenge to detect/quantify an increasing number of possible GMOs at a sustainable cost and affordable
staff effort. Finally, we present our conclusions concerning the applicability of NAIMA for future use in GMO diagnostics. |
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