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Authentication of cassia seeds on the basis of two-wavelength HPLC fingerprinting with the use of chemometrics
Authors:Yan Hua Lai  Yong Nian Ni  Serge Kokot
Institution:a State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China;b Department of Chemistry, Nanchang University, Nanchang, 330031, China;c School of Physical and Chemical Sciences, Queensland University of Technology, Brisbane, Qld 4001, Australia
Abstract:High performance liquid chromatographic(HPLC) fingerprints of Cassia seed,a traditional Chinese medicine(TCM),were developed by means of the chromatograms at two wavelengths of 238 and 282 nm.Then,the two data sets were combined into one matrix.The application of principal component analysis(PCA) for this data matrix showed that the samples were clustered into four groups in accordance with the plant sources and preparation procedures.Furthermore,partial least squares(PLS),back propagation artificial neural...
Keywords:Two-wavelength HPLC fingerprinting  Cassia seeds  Chemometrics  Authentication  
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