Studies on the retention behaviour of some thiazolylazo derivatives in reversed phase liquid chromatography |
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| Authors: | Won Lee Mi-Kyoung Kim Eun-Kyung Kim Kyung-Su Park Young-Sang Kim |
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| Institution: | a Research Institute for Basic Science and Department of Chemistry, Kyunghee University, Seoul 130–701, South Korea;b Division of Liberal Art and Science, Semyung University, Jecheon, Chungbuk 390–711, South Korea;c Advanced Analysis Center, Korea Institute of Science and Technology, Seoul 130–650, South Korea;d Department of Chemistry, Korea University, Jochiwon, Choongnam 339–700, South Korea |
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| Abstract: | The retention behaviour of thiazolylazo derivatives, 4-(2-thiazolylazo) resorcinol (TAR), 4-(2-thiazolylazo)-orcinol (TAO), 2-(2-thiazolylazo)-4-methylphenol (TAC) and 1-(2-thiazolylazo)-2-naphtol (TAN) was studied by reversed phase liquid chromatography. The optimum conditions for the separation of four thiazolylazo derivatives were examined with respect to column, flow rate, mobile phase composition and pH of mobile phase. These derivatives were separated simultaneously on Symmetry C8 column using composition of acetonitrile/water (60/40, v/v) as mobile phase. The capacity factor (k′) has been decreased at higher pH than pKa of solute which may due to the increasing concentration of the ionized species as increase the pH of mobile phase. The dependence of log k′ on the volume faction of water in the binary mobile phase and k′ on the liquid–liquid extraction distribution ratio (Dc) in acetonitrile–water (60/40, v/v)/n-octane extraction system for thiazolylazo derivatives were obtained good linear relationship. The results showed that the retention behaviour of these derivatives was mainly affected by the hydrophobic interaction between thiazolylazo as solute and mobile phase. |
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| Keywords: | Thiazolylazo derivatives RPLC Retention behaviour TAR TAO TAC TAN |
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