Identification of human hepatocellular carcinoma-related proteins by proteomic approaches |
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| Authors: | Ming-Hui Yang Yu-Chang Tyan Shiang-Bin Jong Ying-Fong Huang Pao-Chi Liao Ming-Chen Wang |
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| Affiliation: | (1) Department of Chemistry, Texas Christian University, 2800 S. University Drive, Fort Worth, TX 76129, USA;(2) Department of Environmental and Occupational Health, National Cheng Kung University, 1, Ta-Hsueh Road, Tainan, 701, Taiwan;(3) Department of Nuclear Medicine, Kaohsiung Medical University, 100, Shi-Chuan 1st Road, Kaohsiung, 807, Taiwan;(4) Department of Biomedical Engineering, Yuanpei University, 306, Yuanpei St., Hsinchu, 300, Taiwan;(5) 2200 W Lowden St., Apt. 40, Fort Worth, TX 76110, USA;(6) 154 Hua-Shing St., Yung-Kang, Tainan, Taiwan |
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| Abstract: | ![]()
Hepatocellular carcinoma (HCC) is the most common malignant liver tumor. Analysis of human serum from HCC patients using two-dimensional gel electrophoresis (2DE) combined with nano-high-performance liquid chromatography electrospray ionization tandem mass spectrometry (nano-HPLC–ESI-MS/MS) identified fourteen different proteins differentially expressed between HCC patients and the control group. Twelve proteins were up-regulated and two down-regulated. By using nano-HPLC–MS/MS system to analyze proteome in human serum, 317 proteins were identified, twenty-nine of which to high confidence levels (protein matched at last two unique peptide sequences). Of these twenty-nine proteins, six were present only in HCC patients and may serve as biomarkers for HCC. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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| Keywords: | Proteomics Hepatocellular carcinoma Serum Protein biomarker Mass spectrometry |
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