Selective separation and enrichment of proteins in aqueous two-phase extraction system |
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| Authors: | Feng Qu Hao Qina Min Donga Dong Xu Zhaoa Xin Ying Zhaob Jing Hua Zhangb a School of Life Science Beijing Institute of Technology Beijing China b Beijing Center for Physical Chemical Analysis Beijing China |
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| Institution: | a School of Life Science, Beijing Institute of Technology, Beijing 100081, China;b Beijing Center for Physical and Chemical Analysis, Beijing 100089, China |
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| Abstract: | A simple aqueous two-phase extraction system(ATPS) of PEG/phosphate was proposed for selective separation and enrichment of proteins.The combination of ATPE with HPLC was applied to identify the partition of proteins in two phases.Five proteins (bovine serum albumin,Cytochrome C,lysozyme,myoglobin,and trypsin) were used as model proteins to study the effect of phosphate concentration and pH on proteins partition.The PEG/phosphate system was firstly applied to real human saliva and plasma samples,some protei... |
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| Keywords: | Aqueous two-phase extraction Saliva and plasma Proteins Selective separation and enrichment |
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