| DNA/羟基磷灰石杂化膜修饰电极用于研究DNA与量子点的相互作用 |
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| 引用本文： | 刘燕,李静,马荣娜,赵倩,王国涛,贾文丽,王怀生.DNA/羟基磷灰石杂化膜修饰电极用于研究DNA与量子点的相互作用[J].中国科学:化学,2011,41(1):30-36. |
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| 作者姓名： | 刘燕 李静 马荣娜 赵倩 王国涛 贾文丽 王怀生 |
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| 作者单位： | 聊城大学化学化工学院分析化学研究所, 聊城 252059 |
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| 基金项目： | 本工作得到国家自然科学基金(20475024 & 20775031)和山东省“泰山学者”建设工程专项经费资助 |
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| 摘 要： | 首次利用溶胶-凝胶一步法制备了纳米多孔羟基磷灰石(HAp)-DNA杂化膜修饰玻碳电极,HAp优良的生物相容性和独特的吸附性可以将DNA固定在HAp多孔薄膜上,而DNA的大分子结构对HAp膜起到稳定剂的作用.采用循环伏安法和交流阻抗法系统地研究了电极表面固定DNA的稳定性以及固定的DNA与非电活性核壳型量子点CdTe/C...
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| 关 键 词： | DNA 羟基磷灰石 CdTe/CdS量子点 杂化膜修饰电极 圆二色谱法 |
| 收稿时间： | 2010-01-28 |
| 修稿时间： | 2010-05-05 |
Study on the interaction of DNA with quantum dots using DNA/hydroxyapatite composite film modified electrode |
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| LIU Yan,LI Jing,MA RongNa,ZHAO Qian,WANG GuoTao,JIA WenLi & WANG HuaiSheng College of Chemistry , Chemical Engineering,Liaocheng University,Liaocheng ,China.Study on the interaction of DNA with quantum dots using DNA/hydroxyapatite composite film modified electrode[J].Scientia Sinica Chimica,2011,41(1):30-36. |
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| Authors: | LIU Yan LI Jing MA RongNa ZHAO Qian WANG GuoTao JIA WenLi & WANG HuaiSheng College of Chemistry Chemical Engineering Liaocheng University Liaocheng China |
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| Institution: | LIU Yan,LI Jing,MA RongNa,ZHAO Qian,WANG GuoTao,JIA WenLi & WANG HuaiSheng College of Chemistry and Chemical Engineering,Liaocheng University,Liaocheng 252059,China |
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| Abstract: | Nano-porous hydroxyapatite (HAp)-DNA composite film modified glassy carbon electrode was prepared by sol-gel one-step method for the first time. DNA was immobilized on the nano-porous HAp film firmly due to its effective adsorbability and biocompatibility and the structure of macromolecule of DNA plays an important role in the stabilization of HAp film. Studies on the interaction of DNA with non-electroactive core-shell CdTe/CdS quantum dots were carried out using the DNA/HAp modified electrode by cyclic voltammetry and impedance analysis. The results showed that DNA immobilized on the HAp film was stably enough for the electrochemical studies and there exists a remarkable intercalative interaction mode between QDs and DNA and the intercalative interaction could influence the configuration of DNA. This studies can provide important theoretical basis for the study on the toxicity of QDs and a new thinking for the study on the interaction of DNA with non-electroactive molecules using electrochemical methold. |
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| Keywords: | DNAzz hydroxyapatitezz CdTe/CdS quantum dotszz composite film modified electrodezz circular dichroismzz |
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