纳升电喷雾萃取电离质谱快速测定人参皂苷

以自行研制的纳升电喷雾萃取电离源（NanoEESI）为基础，建立了在无需色谱分离条件下快速测定人参皂苷的质谱分析方法，用NanoEESI串联质谱分析鉴定了人参中的多种人参皂苷，并将该方法用于人参、桔梗和商陆的快速鉴定。实验表明，人参中的皂苷易于在电离过程中结合钠离子，并形成M+Na]+正离子从而被检测分析，而桔梗和商陆等样品中不含人参皂苷成份，在串联质谱中也没有相应的特征碎片离子，从而达到鉴别中药材真伪的目的。本方法简便、快速、灵敏、特征性强、重现性好，对道地中药材快速鉴定提供了思路，具有很好的实用价值。

Rapid Detection of Ginsenosides Using NanoEESI-MS

A rapid approach to detect ginsenosides has been developed based on nano electrospray extractive ionization (NanoEESI) tandem mass spectrometry. The raw extract of ginseng roots were directly introduced to the gasless nanoEESI source for production of sodiated ions of ginsenosides, without any chromatographic separation. The signals of sinsenosides M+Na]+ were identified by tandem mass spectrometry. The characteristic fragmentation patterns were therefore used as the molecular markers to differentiate ginseng roots from platycodon grandiflorum and phytolacca acinosa rox, where are two typical plant roots illicitly used as ginseng roots on the herb medicine market. Acceptable sensitivity, reproducibility and fast speed were obtained for the samples tested, showing that nano extractive electrospray ionization tandem mass spectrometry is a useful technique for fast screening ginseng products or other traditional Chinese medicinal materials.