| 胶原-磺化羧甲基壳聚糖/硅橡胶皮肤再生材料的制备及其对小型猪烫伤创面全层皮肤缺损的修复研究 |
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| 引用本文： | 黄爱宾,郭瑞,徐少骏,马列,高长有.胶原-磺化羧甲基壳聚糖/硅橡胶皮肤再生材料的制备及其对小型猪烫伤创面全层皮肤缺损的修复研究[J].高分子学报,2009,0(2):111-117. |
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| 作者姓名： | 黄爱宾 郭瑞 徐少骏 马列 高长有 |
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| 作者单位： | 1. 教育部高分子合成与功能构造重点实验室,浙江大学高分子科学与工程学系,杭州,310027
2. 杭州师范大学临床医学院,杭州,310036 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)，浙江省科技计划项目，国家杰出青年科学基金 |
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| 摘 要： | 制备了一种胶原-磺化羧甲基壳聚糖/硅橡胶皮肤再生材料,并以小型猪为模型,考察了其对烫伤全层皮肤缺损的修复性能.首先合成了磺化羧甲基壳聚糖,并对其结构进行了表征.制备了胶原-磺化羧甲基壳聚糖多孔支架,采用扫描电子显微镜(SEM)研究了磺化羧甲基壳聚糖含量对支架微结构的影响.随着磺化羧甲基壳聚糖含量的增大,胶原-磺化羧甲基壳聚糖支架从纤维结构向片状结构转化,且支架的孔径相对变大.采用体外成纤维细胞培养实验证明胶原-磺化羧甲基壳聚糖支架无明显细胞毒性.进一步将胶原-磺化羧甲基壳聚糖支架与硅橡胶膜复合,构建具有双层结构的皮肤再生材料.以小型猪为模型,评价了其对深度烫伤创面的修复性能.大体观察和组织学分析结果显示,胶原-磺化羧甲基壳聚糖/硅橡胶皮肤再生材料具有更快的血管化性能,且经该材料处理的创面能有效支持薄自体皮片的移植成活,实现深度烫伤创面的全层修复.
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| 关 键 词： | 磺化羧甲基壳聚糖 胶原 皮肤再生材料 烫伤 血管化 |
| 收稿时间： | 2008-09-26 |
PREPARATION OF COLLAGEN-SULFONATED CARBOXYMETHYL CHITOSAN/SILICONE MEMBRANE BILAYER DERMAL EQUIVALENT AND ITS APPLICATION IN THE TREATMENT OF SCALDS OF PORCINE SKIN |
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| HUANG Aibin,GUO Rui,XU Shaojun,MA Lie,GAO Changyou.PREPARATION OF COLLAGEN-SULFONATED CARBOXYMETHYL CHITOSAN/SILICONE MEMBRANE BILAYER DERMAL EQUIVALENT AND ITS APPLICATION IN THE TREATMENT OF SCALDS OF PORCINE SKIN[J].Acta Polymerica Sinica,2009,0(2):111-117. |
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| Authors: | HUANG Aibin GUO Rui XU Shaojun MA Lie GAO Changyou |
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| Institution: | 1 Key Laboratory of Macromolecular Synthesis and Functionalization;Ministry of Education;Department of Polymer Science and Engineering;Zhejiang University;Hangzhou 310027;2 College of Clinical Medical;Hangzhou Normal University;Hangzhou 310036 |
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| Abstract: | A dermal equivalent applied in the treatment of scalds has been attracting much attention.We report here the fabrication of a bilayer dermal equivalent(BDE) composed of collagen-sulfonated carboxymethyl chitosan(SCC) porous scaffold and silicone membrane,and its application for accelerating the healing of skin scalds in a Bama miniature pig model.Firstly,the SCC was synthesized,and its chemical structure was characterized by FTIR and ~1H-NMR.Then the effect of the ratio of the SCC on the microstructure of t... |
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| Keywords: | Sulfonated carboxymethyl chitosan Collagen Dermal equivalent Scald Angiogenesis |
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