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Developments in the production of biological and synthetic binders for immunoassay and sensor-based detection of small molecules |
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| Authors: | Terry FodeyPaul Leonard John O’MahonyRichard O’Kennedy Martin Danaher |
| Institution: | a Institute of Agri-Food and Land Use, School of Biological Sciences, Queen’s University Belfast, Belfast, Ireland b School of Biotechnology and National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland c Biomedical Diagnostics Institute, Dublin City University, Dublin 9, Ireland d Teagasc, Ashtown Food Research Centre, Ashtown, Dublin 15, Ireland |
| Abstract: | The need for chemical and biological entities of predetermined selectivity and affinity towards target analytes is greater than ever, in applications such as environmental monitoring, bioterrorism detection and analysis of natural toxin contaminants in the food chain.In this review, we focus on advances in the production of specific binders, in terms of both natural entities (e.g., antibodies) and synthetic binders (e.g., molecularly-imprinted polymers). We discuss the potential of emerging technologies for integration into immunoassay and sensing techniques. We place special emphasis on use of these technologies in bioanalytical applications. |
| Keywords: | Antibody Aptamer Binding protein Biorecognition Immunoassay Molecular-imprinted polymer Nanobody Protein scaffold Single-chain variable fragment Synthetic binder |
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