Electrochemical investigation of platinum-coated gold nanoporous film and its application for Escherichia coli rapid measurement |
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Authors: | Qinyan Yang Ying Liang Tianshu Zhou Guoyue Shi Litong Jin |
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Institution: | 1. Department of Chemistry and Shanghai Key Laboratory of Green Chemistry and Chemical Process, East China Normal University, 3663 Zhongshan Road(N), Shanghai 200062, PR China;2. Department of Environmental Science, East China Normal University, 3663 Zhongshan Road(N), Shanghai 200062, PR China |
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Abstract: | Pt-nanoparticle-coated gold nanoporous film (PGNF) was synthesized via a simple nonpolluting approach and PGNF modified electrode was also constructed successfully for the rapid measurement of Escherichia coli (E. coli) in this work. Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) images showed that the resulting PGNF electrode had highly ordered arrangement and large surface area. Furthermore, the electrochemical characteristics of the PGNF electrode were investigated by cyclic voltammetry (CV) and amperometric i-t curve. The PGNF electrode showed excellent electrocatalytic activity to E. coli and the current responses were in good linear from 2 × 101 cfu/ml to 1 × 106 cfu/ml with the detection limit of 10 cfu/ml (S/N = 3) without pretreatment. The high sensitivity, wider linear range and good reproducibility make this PGNF a promising candidate for portable amperometric E. coli sensor. |
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