Electrochemical Sensors Based on Stationary Electrodes and Immobilized DNA or Its Fragments and the Assessment of Their Analytical Potentials

Amperometric biosensors were developed on the basis of stationary mercury-film and glassy-carbon electrodes and DNA or its fragments, oligodeoxynucleotides (ODNs), immobilized in a nitrocellulose matrix. Taking into account the high affinity of Cu(II) and Fe(III) ions to denatured DNA ((19.1 ± 0.1) ×10⁵ and (1.4 ± 0.3) × 10⁵ L/mol, respectively), a procedure was proposed for the voltammetric determination of these ions in natural materials and blood serum at a level of n × 10⁻¹¹ M. This procedure involves analyte pre-concentration on a DNA-containing biosensor. An ODN-containing biosensor (DNA probe) was used in the study of DNA hybridization for the highly specific determination of its nucleotide sequence.__________Translated from Zhurnal Analiticheskoi Khimii, Vol. 60, No. 6, 2005, pp. 639–645.Original Russian Text Copyright © 2005 by Babkina, Palecek, Jelen, Fojta.Presented at the VI All-Russia Conference (with international participation) on Electrochemical Methods of Analysis (EMA-2004, Ufa, May 23–27, 2004).