Improved Performance of Lipase Immobilized on Tannic Acid-Templated Mesoporous Silica Nanoparticles |
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Authors: | Yanjun Jiang Wenya Sun Liya Zhou Li Ma Ying He Jing Gao |
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Institution: | 1.School of Chemical Engineering and Technology,Hebei University of Technology,Tianjin,China |
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Abstract: | Mesoporous silica nanoparticles were synthesized by using tannic acid as a pore-forming agent, which is an environmentally friendly, cheap, and non-surfactant template. SEM and TEM images indicated that the tannic acid-templated mesoporous silica nanoparticles (TA-MSNs) are monodisperse spherical-like particles with an average diameter of 195?±?16 nm. The Brunauer–Emmett–Teller (BET) results showed that the TA-MSNs had a relatively high surface area (447 m2/g) and large pore volume (0.91 cm3/g), and the mean pore size was ca. 10.1 nm. Burkholderia cepacia lipase was immobilized on the TA-MSNs by physical adsorption for the first time, and the properties of immobilized lipase (BCL@TA-MSNs) were investigated. The BCL@TA-MSNs exhibited satisfactory thermal stability; strong tolerance to organic solvents such as methanol, ethanol, isooctane, n-hexane, and tetrahydrofuran; and high operational reusability when BCL@TA-MSNs were applied in esterification and transesterification reactions. After recycling 15 times in the transesterification reaction for biodiesel production, over 85 % of biodiesel yield can be maintained. With these desired characteristics, the TA-MSNs may provide excellent candidates for enzyme immobilization. |
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