Fluorimetric determination of ascorbic acid with o-phenylenediamine |
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Authors: | Wu Xia Diao Yuxia Sun Changxia Yang Jinghe Wang Yuebo Sun Shuna |
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Institution: | Key Laboratory for Colloid and Interface Chemistry of Education Ministry, Department of Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China |
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Abstract: | A simple and sensitive fluorimetric method for the determination of ascorbic acid (AA) is described. The method is based on the condensation reaction between AA and o-phenylenediamine (OPDA) in the absence of the oxidant. The fluorescence intensity is measured at excitation and emission wavelengths of 360 and 430 nm, respectively. Under optimum condition, a linear relationship is obtained between the fluorescence intensity and the concentration of AA in the range of 0.05-40 μg ml−1. The detection limit is 0.006 μg ml−1, which is obviously lower than that of other fluorimetric methods reported. |
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Keywords: | Fluorimetric determination Ascorbic acid (AA) o-Phenylenediamine (OPDA) |
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