Determination of L- and D-enantiomers of methotrexate using amperometric biosensors |
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| Authors: | Stefan Raluca-Ioana Bokretsion Rahel Girmai van Staden Jacobus F Aboul-Enein Hassan Y |
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| Affiliation: | a Department of Chemistry, University of Pretoria, 0002 Pretoria, South Africa
b Pharmaceutical Analysis and Drug Development Laboratory, Biological and Medical Research Department (MBC-03-65), King Faisal Specialist Hospital and Research Center, PO Box 3354, Riyadh 11211, Saudi Arabia |
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| Abstract: | In order to determine the enantiopurity of methotrexate (Mtx), seven biosensors were proposed for the assay of l-Mtx and three biosensors for the assay of d-Mtx. The biosensors were designed using physical and chemical immobilization of glutamate oxidase and/or l-amino acid oxidase (l-AAOD) and/or horseradish peroxidase (HRP) for the assay of l-methotherexate, and d-amino acid oxidase (d-AAOD) and HRP for the assay of d-Mtx. Electrode characteristics were obtained and compared for the different carbon paste based biosensors. The linear concentration ranges for the proposed biosensors were in the ranges of fmol l−1 to pmol l−1, magnitude order with limits of detection in the fmol l−1 to nmol l−1 concentration range. All biosensors were successful for the determination of the enantiopurity of Mtx as raw material, and in its pharmaceutical formulations (tablets and injections). |
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| Keywords: | Amperometric biosensor smallcaps" >l( smallcaps" >d)-Mtx smallcaps" >l( smallcaps" >d)-Amino acid oxidase Horseradish peroxidase Glutamate oxidase |
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