Multidetermination of thiamine HCl and pyridoxine HCl in their mixture using continuous daubechies and biorthogonal wavelet analysis

A new graphical method based on the one-dimensional wavelet transform (WT) was proposed and tested on mixture of thiamine hydrochloride (THI) and pyridoxine hydrochloride (PYR) in the presence of strongly overlapping signals. We selected from the data of the UV-VIS absorption spectra a signal consisting of 1150 points corresponding to the concentration range 8-32 mg ml⁻¹ for each vitamin and we subjected it to Daubechies8 (DAUB8) and Biorthogonal6.8 (BIOR6.8) wavelet transforms. Since the peaks of the transformed signals were bigger than original ones a zero crossing method was applied to obtain the calibration graphs. In addition, the validity of Beer-Lambert law was assumed for the transformed signals. An appropriate scale setting was choosing to obtain an alternative calibration for each method. Matlab 6.5 software was used for one-dimensional wavelet analysis and the basic concepts about wavelet method were given. The obtained results were successfully compared among each other as well as with those obtained by other literature methods. The method developed in this paper is rapid, easy to apply, not expensive and it is suitable for analyzing of the overlapping signals of compounds in their mixtures without any chemical pre-treatment.