Nonaqueous CE for Rapid and Sensitive Determination of Matrine and Oxymatrine in Sophora flavescens and Its Medicinal Preparations |
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Authors: | Qinhua Chen Peng Li Fanjun Cheng Bing Li Songchao Wu and Jing He |
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Institution: | (1) Department of Pharmacy, Affiliated Dongfeng General Hospital, Yunyang Medical College, 442008 Hubei, People’s Republic of China |
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Abstract: | A simple, rapid, and sensitive non-aqueous capillary electrophoresis procedure for the quantitative determination of matrine
and oxymatrine is established. Optimum separation conditions were obtained when the sample was injected under pressure for
3 s at 50 mbar and separated with the buffer containing 70 mM ammonium acetate, 7.0% (v/v) acetic acid, and 10% (v/v) acetonitrile in methanol medium at 25 kV applied voltage. The analytes were detected at 205 nm. The two alkaloids can be
separated within 12 min and quantified with high sensitivity. The method was validated in terms of reproducibility, linearity,
and accuracy when applied to the analysis of matrine and oxymatrine in Sophora flavescens and its medicinal preparations. |
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