Determination of catecholamines and serotonin by micellar electrokinetic chromatography with laser-induced fluorescence detection |
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Authors: | Cao Li-Wei |
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Institution: | Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China. lwcao@126.com |
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Abstract: | 6-Oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl) fluorescein, a new synthesized fluorescent reagent, was established for the first time as a label for the sensitive analysis of catecholamines (CAs) and serotonin (5-HT) by micellar electrokinetic chromatography (MEKC) with laser-induced fluorescence detection. After careful study on the derivatization conditions such as pH value, reagent concentration, temperature and reaction time, the labeling reaction was accomplished as quickly as 7 min with stable yield. The separation parameters for the CAs and 5-HT were also optimized in detail. The derivatives were baseline separated in a running buffer containing 30 mM boric acid and 15 mM sodium dodeculsulfate at pH 9.0. The detection limits ranged from 5 x 10(-10) to 2 x 10(-9) M (signal-to-noise ratio = 3). The rapid and sensitive method was also applied to the determination of the CAs and 5-HT of urine samples. |
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Keywords: | 6‐Oxy‐(N‐succinimidyl acetate)‐9‐(2′‐methoxycarbonyl) fluorescein catecholamine serotinin derivatization capillary electrophoresis laser‐induced fluorescence detection |
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