磷酸化肽段分离富集方法研究进展

目前磷酸化肽段鉴定主要依赖于质谱技术,但磷酸化肽段的低丰度性以及来自非磷酸化肽段的干扰等因素,影响质谱的分析与鉴定。因此质谱分析前磷酸化肽段的富集,是深入研究磷酸化蛋白质组学的先决条件。该文介绍了磷酸化蛋白质组学中传统的以及新建立的一些磷酸化肽段分离富集方法的原理及优缺点,这些方法包括固相金属离子亲和色谱法(IMAC)、金属氧化亲和色谱法(MOAC)、强阳/阴离子交换色谱法(SCX/SAX)、亲水相互作用色谱法(HILIC)、静电排斥亲水相互作用色谱法(ERLIC)、化学衍生法、MALDI靶盘富集法以及多种富集方法相结合。

Research Advances in Separation and Enrichment Approaches for Phosphopeptides

Currently,the identification of phosphopeptides mainly depends on mass spectrometry,but the low abundance of phosphopeptide and the interference of non phosphopeptide affect the analysis and identification of mass spectrometry.Therefore,the enrichment of phosphopeptide before mass spectrometry is a prerequisite for further study of phosphoproteomics.This review summarizes the mechanisms and advantages or disadvantages of those traditional and new developed methods for phosphopeptides enrichment,including immobilized metal ion affinity chromatography(IMAC), metal oxide affinity chromatography(MOAC), strong cation/anion exchange chromatography(SCX/SAX), hydrophilic interaction chromatography(HILIC), ectrostatic repulsion-hydrophilic interaction chromatography(ERLIC), chemical derivatization, matrix assisted laser desorption/ionization(MALDI) plate specific enrichment method, and coupled methods.