Sensitive Near-Infrared Fluorescent Probes for Thiols Based on Se?N Bond Cleavage: Imaging in Living Cells and Tissues |
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| Authors: | Rui Wang Prof Lingxin Chen Ping Liu Qin Zhang Yunqing Wang |
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| Institution: | 1. Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai Shandong 264003, 17 Chunhui Road, Yantai 264003 (P.R. China), Fax: (+86) 535-2109130;2. Linshu People's Hospital of Shandong Province, Linshu 276700 (P.R. China) |
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| Abstract: | Cy-NiSe and Cy-TfSe were designed and synthesized as sensitive near-infrared (NIR) fluorescent probes for detecting thiols on the basis of Se N bond cleavage both in cells and in tissues. Since a donor-excited photoinduced electron transfer (d-PET) process occurs between the modulator and the fluorophore, Cy-NiSe and Cy-TfSe have weak fluorescence. On titration with glutathione, the free dye exhibits significant fluorescence enhancement. The two probes are sensitive and selective for thiols over other relevant biological species. They can function rapidly at pH 7.4, and their emission lies in the NIR region. Confocal imaging confirms that Cy-NiSe and Cy-TfSe can be used for detecting thiols in living cells and tissues. |
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| Keywords: | cleavage reactions fluorescent probes imaging agents Se?N bonds thiols |
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