| 鲨鱼软骨血管抑制因子的高效液相色谱与电喷雾质谱研究 |
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| 引用本文： | 岳贵花,杨芃原,沈先荣,吉冬梅,任大明,刘为平,许云敏,王洪海.鲨鱼软骨血管抑制因子的高效液相色谱与电喷雾质谱研究[J].高等学校化学学报,1999,20(12):1868-1870. |
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| 作者姓名： | 岳贵花 杨芃原 沈先荣 吉冬梅 任大明 刘为平 许云敏 王洪海 |
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| 作者单位： | 1. 复旦大学化学系, 上海 200433;
2. 复旦大学遗传所, 上海 200433 |
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| 基金项目： | 国家教育部科技重点项目基金，博士点基金!(批准号 :98024614)，复旦大学资助课题 |
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| 摘 要： | 电喷雾质谱 ( ESIMS)具有快速、灵敏等特点 ,近年来已成为鉴定和分析多肽、蛋白质和核酸的有力工具 .将 ESIMS与高效液相色谱 ( HPLC)联用 ,在蛋白质分子量、结构及活性位点等方面的研究已取得较大的进展 1 ] .鲨鱼软骨血管抑制因子 ( SCAIF- I)是作者之一从鲨鱼软骨中提取的一种未知蛋白质2 ] ,研究表明 ,SCAIF- I对肿瘤、癌症的抑制及治疗具有明显的疗效3] .因此 ,对其结构的研究具有重要的意义 .本文报道了用 HPL C与 ESIMS对未知蛋白质 SCAIF- I的分子量及肽段部分序列的测定结果 .1 实验部分1 .1 样品制备 SC…
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| 关 键 词： | 高效液相色谱 电喷雾质谱 鲨鱼软骨血管抑制因子 |
| 收稿时间： | 1999-08-24 |
Identification of Angiogenesis Inhibitory Factor by HPLC and ESIMS |
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| YUE Gui-hua,YANG Peng-Yuan,SHEN Xian-rong,JI Dong-mei,REN Da-ming,LIU Wei-Ping,XU Yun-Min,WANG Hong-hai.Identification of Angiogenesis Inhibitory Factor by HPLC and ESIMS[J].Chemical Research In Chinese Universities,1999,20(12):1868-1870. |
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| Authors: | YUE Gui-hua YANG Peng-Yuan SHEN Xian-rong JI Dong-mei REN Da-ming LIU Wei-Ping XU Yun-Min WANG Hong-hai |
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| Institution: | 1. Department of Chemistry, Fudan University, Shanghai 200433, China;
2. Institute of Genetic, Fudan University, Shanghai 200433, China |
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| Abstract: | The studies of SCAIF I(an unknown protein) by HPLC and ESIMS was reported in this paper. The SCAIF I is a kind of antiangiogenesis extracted from shark cartilage. The extracted protein was purified by HPLC and then studied by ESIMS. The molecule weight of the SCAIF I protein was confirmed to be 15 601 in the first time. Peptide map of the unknown protein was obtained by HPLC ESIMS with a trypsin digestion and possible partial sequences were deduced with the online CID technique. With partial sequence information the homologous proteins are searched with protein database. The homology of SCAIF I summarized in this work is useful in further studies of this unknown protein. |
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| Keywords: | HPLC ESIMS SCAIF |
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