The Influence of Solvent Viscosity on the Fluorescence Decay and Time-Resolved Anisotropy of Green Fluorescent Protein |
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| Authors: | Klaus Suhling Daniel M. Davis David Phillips |
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| Affiliation: | (1) Department of Biological Sciences, Sir Alexander Fleming Building, Imperial College of Science, Technology & Medicine, London, SW7 2AZ, U.K.;(2) Department of Chemistry, Imperial College of Science, Technology & Medicine, London, SW7 2AZ, U.K |
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| Abstract: | ![]()
This report describes fluorescence decay and time-resolved anisotropy studies of green fluorescent protein (GFP) in various environments. The addition of glucose and fructose, NaCl, or polyethylene glycol changes the viscosity of the medium surrounding the GFP. Both the time-resolved anisotropy and the fluorescence decay of GFP are measured and it is shown that only the time-resolved anisotropy of GFP is affected by the viscosity, but not its fluorescence decay. |
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| Keywords: | GFP fluorescence lifetime anisotropy TCSPC viscosity |
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