Surface characterization of a cross‐linked cytochrome c film on cysteamine‐modified gold electrodes |
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| Authors: | Karolien De Wael Sandra Van Vlierberghe Hans Buschop Peter Dubruel Bart Vekemans Etienne Schacht Laszlo Vincze Annemie Adriaens |
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| Institution: | 1. Department of Analytical Chemistry, Ghent University, Krijgslaan 281 S12, B‐9000 Ghent, Belgium;2. Postdoctoral Fellow of the Research Foundation ‐ Flanders (Belgium).;3. Polymer Chemistry and Biomaterials Research Group, Ghent University, Krijgslaan 281 S4, B‐9000 Ghent, Belgium |
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| Abstract: | The aim of the present paper is to characterize a cross‐linked horse heart cytochrome c (HHC) film on cysteamine‐modified gold electrodes. The HHC film was deposited using 1‐ethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide (EDC) as a coupling agent. Attenuated total reflection infrared (ATR‐IR) spectroscopic analyses were performed to characterize the newly formed surface on a qualitative and conformational level. The film thickness was measured using a noncontact optical surface profiler, while quantitative data and information on the heterogeneity of the film were obtained by means of synchrotron radiation X‐ray micro fluorescence (SR micro‐XRF). Results indicate that, in addition to electrochemical studies, spectroscopic analysis methods are essential to gain insight in the effect of immobilization strategies on protein conformations. The latter is of relevance in the development and optimization of biosensors. Copyright © 2009 John Wiley & Sons, Ltd. |
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| Keywords: | cytochrome c EDC cross‐linking ATR‐IR spectroscopy synchrotron radiation micro X‐ray fluorescence surface profilometry bioelectrochemistry biosensor |
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