Phosphopeptide enrichment with stable spatial coordination on a titanium dioxide coated glass slide |
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| Authors: | Susumu Y Imanishi Petri Kouvonen Jan‐Henrik Smått Mikko Heikkilä Emilia Peuhu Andrey Mikhailov Mikko Ritala Mika Lindén Garry L Corthals John E Eriksson |
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| Institution: | 1. Turku Centre for Biotechnology, University of Turku and ?bo Akademi University, FIN‐20521 Turku, Finland;2. Department of Biology, ?bo Akademi University, FIN‐20520 Turku, Finland;3. Center for Functional Materials, Department of Physical Chemistry, ?bo Akademi University, FIN‐20500 Turku, Finland;4. Laboratory of Inorganic Chemistry, Department of Chemistry, University of Helsinki, FIN‐00014 Helsinki, Finland |
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| Abstract: | Recent advances in phosphoproteomics have established powerful tools to analyze phosphorylation events. However, their spatial localization is lost due to sample homogenization procedures prior to the analysis. Imaging mass spectrometry (IMS) has emerged as a method to visualize the spatial distribution of molecules in tissue samples, but its application is still limited to relatively abundant molecules. Due to low phosphorylation stoichiometry, direct detection and imaging of protein phosphorylation by MS has not been achieved yet. Therefore we have developed a novel phosphopeptide enrichment strategy as a potential tool for in situ affinity imaging MS (AIMS). A specific type of titanium dioxide (TiO2)‐coated glass slides was designed and validated with casein tryptic digests for their ability to selectively retain phosphopeptides while maintaining their spatial coordination. Copyright © 2009 John Wiley & Sons, Ltd. |
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