Profile analysis of organic volatiles in urine of tobacco smoke-exposed beagles

Volatile organic components were determined in the urine of beagle dogs exposed to cigarette smoke and sham in an effort to identify possible biochemical changes resulting from the exposures. The data obtained from high-resolution chromatograms were subjected to principal component and discriminant analysis. Principal component analysis was used to reduce the dimensionality of the data and discriminant analysis was used to develop a classification model. Classification of the profiles into their respective exposure groups based on quantitative variations was 92% correct using only five peaks in the chromatograms. Compounds present in the urine of smoke-exposed dogs were identified by mass spectrometry.