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Evaluation of the effects of Candidatus Liberibacter asiaticus on inoculated citrus plants using laser-induced breakdown spectroscopy (LIBS) and chemometrics tools |
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| Authors: | Pereira Fabíola Manhas Verbi Milori Débora Marcondes Bastos Pereira Venâncio André Leonardo Russo Mariana de Sá Tavares Martins Polyana Kelly Freitas-Astúa Juliana |
| Affiliation: | a Embrapa Instrumentação Agropecuária, P.O. Box 741, Zip Code 13561-206, São Carlos, SP, Brazil b Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Departamento de Física e Matemática, Universidade de São Paulo, Av. Bandeirantes, Zip Code 14040-901, 3900 Ribeirão Preto, SP, Brazil c Centro Apta Citros Sylvio Moreira, P.O. Box 04, Zip Code 13490-970, Cordeirópolis, SP, Brazil d Embrapa Mandioca e Fruticultura Tropical, R. Embrapa, s/n, Zip Code 44380-000, Cruz das Almas, BA, Brazil |
| Abstract: | This study investigated the organic and inorganic constituents of healthy leaves and Candidatus Liberibacter asiaticus (CLas)-inoculated leaves of citrus plants. The bacteria CLas are one of the causal agents of citrus greening (or Huanglongbing) and its effect on citrus leaves was investigated using laser-induced breakdown spectroscopy (LIBS) combined with chemometrics. The information obtained from the LIBS spectra profiles with chemometrics analysis was promising for the construction of predictive models to identify healthy and infected plants. The major, macro- and microconstituents were relevant for differentiation of the sample conditions. The models were then applied to different inoculation times (from 1 to 8 months). The models were effective in the classification of 82-97% of the diseased samples with a 95% significance level. The novelty of this method was in the fingerprinting of healthy and diseased plants based on their organic and inorganic contents. |
| Keywords: | Laser-induced breakdown spectroscopy Chemometrics Fingerprint Plant material Citrus greening Huanglongbing |
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