4D cardiac imaging at clinical 3.0 T provides accurate assessment of murine myocardial function and viability |
| |
| Institution: | 1. Division of Radiology, Department of Radiology and Medical Informatics, Geneva University Hospital and Faculty of Medicine, University of Geneva, 4 rue Gabrielle-Perret-Gentil, 1205 Geneva, Switzerland;2. First Clinic of Internal Medicine, Department of Internal Medicine, University of Genoa, 6 viale Benedetto XV, 16132 Genoa, Italy;3. IRCCS AOU San Martino - IST, Genova, 10 Largo Rosanna Benzi, 16132 Genoa, Italy;4. Division of Pathology, Department of Genetics and Laboratory Medicine, Geneva University Hospitals, 4 rue Gabrielle-Perret-Gentil, 1205 Geneva, Switzerland;5. Division of Cardiology, Foundation for Medical Researches, Faculty of Medicine, Department of Internal Medicine, University of Geneva, 64 avenue de la Roseraie, 1211 Geneva, Switzerland;1. Laboratory of Clinical Investigation, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA;2. Clinical Research Core, Office of the Scientific Director, National Institute on Aging, National Institutes of Health, Baltimore, MD 21225, USA;1. Changchun Institute of Applied Chemistry Chinese Academy of Sciences, No. 5625, Renmin Street, Changchun 130022, China;2. University of Chinese Academy of Sciences, No. 19, Yuquan Road 19, Beijing 100049, China;1. Division of Mathematical oncology, City of Hope National Medical Center, CA, USA;2. Department of Radiology and Imaging, Fortis Memorial Research Institute, Gurgaon, India;3. Department of Biostatistics and Health Informatics, SGPGIMS, Lucknow, India;4. Department of Neurosurgery, Fortis Memorial Research Institute, Gurgaon, India;5. SRL Diagnostics, Fortis Memorial Research Institute, Gurgaon, India;6. Philips Health System, Philips India Limited, Gurgaon, India;1. Department of Reparative Materials, Institute for Frontier Medical Sciences, Kyoto University, Japan;2. Department of Neurosurgery, Jikei University School of Medicine, Japan;3. Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Japan;4. Hiratsuka Technical Center, Tanaka Kikinzoku Kogyo K.K., Japan;5. Isehara Technical Center, Tanaka Kikinzoku Kogyo K.K., Japan |
| |
| Abstract: | ObjectivesWe validate a 4D strategy tailored for 3 T clinical systems to simultaneously quantify function and infarct size in wild type mice after ischemia/reperfusion, with improved spatial and temporal resolution by comparison to previous published protocols using clinical field MRI systems.MethodsC57BL/6J mice underwent 60 min ischemia/reperfusion (n = 14) or were controls without surgery (n = 6). Twenty-four hours after surgery mice were imaged with gadolinium injection and sacrificed for post-mortem MRI and histology with serum also taken for Troponin I levels. The double ECG- and respiratory-triggered 3D FLASH (Fast Low Angle Shot) gradient echo (GRE) cine sequence had an acquired isotropic resolution of 344 μm, TR/TE of 7.8/2.9 ms and acquisition time 25–35 min. The conventional 2D FLASH cine sequence had the same in-plane resolution of 344 μm, 1 mm slice thickness and TR/TE 11/5.4 ms for an acquisition time of 20–25 min plus 5 min for planning. Left ventricle (LV) and right ventricle (RV) volumes were measured and functional parameters compared 2D to 3D, left to right and for inter and intra observer reproducibility. MRI infarct volume was compared to histology.ResultsFor the function evaluation, the 3D cine outperformed 2D cine for spatial and temporal resolution. Protocol time for the two methods was equivalent (25–35 min). Flow artifacts were reduced (p = 0.008) and epi/endo-cardial delineation showed good intra and interobserver reproducibility. Paired t-test comparing ejection volume left to right showed no significant difference for 3D (p = 0.37), nor 2D (p = 0.30) and correlation slopes of left to right EV were 1.17 (R2 = 0.75) for 2D and 1.05 (R2 = 0.50) for 3D.Quantifiable ‘late gadolinium enhancement’ infarct volume was seen only with the 3D cine and correlated to histology (R2 = 0.89). Left ejection fraction and MRI-measured infarct volume correlated (R2 > 0.3).ConclusionsThe 4D strategy, with contrast injection, was validated in mice for function and infarct quantification from a single scan with minimal slice planning. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
