Intravoxel incoherent motion and diffusion tensor imaging of early renal fibrosis induced in a murine model of streptozotocin induced diabetes |
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| Institution: | 1. Department of Oncologic Imaging, National Cancer Centre, Singapore;2. Department of Renal Medicine, General Hospital, Singapore, Singapore;3. Division of Gastroenterology & Hepatology, National University Hospital, Singapore;4. Department of Diagnostic Radiology, Singapore General Hospital, Singapore;5. Roche-Singapore Hub for Translational Medicine, Singapore;6. Royal Marsden Hospital, Surrey, UK;7. Singapore Bioimaging Consortium, Singapore;8. Department of Pathology, Singapore General Hospital, Singapore;1. Department of Nephrology and Hypertension, CHUV, Lausanne, Switzerland;2. Department of Radiology, Medical University of Gdansk, Gdansk, Poland;3. Department of Radiology, CHUV, Lausanne, Switzerland;4. Department of Hypertension and Diabetology, Medical University of Gdansk, Poland;5. Department of Nephrology and Hypertension, Bern University Hospital, Bern, Switzerland;1. Department of Medicine, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA;2. Department of Radiology, Medical Physics, Medical Center University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany;3. Maastricht Brain Imaging Centre, Faculty of Psychology & Neuroscience, Maastricht University, Netherlands;1. Department of Radiology, Shengjing Hospital of China Medical University, Shenyang 110004, PR China;2. Philips Healthcare, 7F, Tower No. 2, The World Profit Centre, No.16 Tianze Road, Chaoyang District, Beijing 100600, PR China |
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| Abstract: | IntroductionTo assess if parameters in intravoxel incoherent motion (IVIM) and diffusion tensor imaging (DTI) can be used to evaluate early renal fibrosis in a mouse model of diabetic nephropathy.Materials & methodsIn a population of 38 male CD1 mice (8 weeks old, 20–30 g), streptozotocin induced diabetes was created in 20 mice via a single intraperitoneal injection of streptozotocin at 150 mg/kg, while 18 mice served as control group. IVIM parameters were acquired at 0, 12 and 24 weeks after injection of streptozotocin using a range of b values from 0 to 1200 s/mm2. DTI parameters were obtained using 12 diffusion directions and lower b values of 0, 100 and 400 s/mm2. DTI and IVIM parameters were obtained using region of interests drawn over the renal parenchyma. Histopathological analysis of the right kidney was performed in all mice. Results were analyzed using an unpaired t-test with P < 0.05 considered statistically significant.ResultsRenal cortex fractional anisotropy (FA) was significantly lower in the diabetes group at week 12 as compared with the control group. Renal cortex apparent diffusion coefficient and tissue diffusivity were significantly higher in the diabetes group at week 12 compared with the control group at 12 weeks. Blood flow was significantly decreased at the renal medulla at 24 weeks. Histopathological analysis confirmed fibrosis in the diabetes group at 24 weeks.ConclusionFA is significantly reduced in diabetic nephropathy. FA might serve a potential role in the detection and therapy monitoring of early diabetic nephropathy. |
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