| 反相高效液相色谱法用于葛根黄酮提取物的分离与主要活性成分的测定 |
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| 引用本文： | 李梅青,盛旋,邵学广.反相高效液相色谱法用于葛根黄酮提取物的分离与主要活性成分的测定[J].分析化学,2003,31(2):178-180. |
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| 作者姓名： | 李梅青 盛旋 邵学广 |
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| 作者单位： | 1. 中国科学技术大学化学系,合肥,230026
2. 安徽出入境检验检疫局,合肥,230061 |
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| 摘 要： | 采用反相高效液相色谱法，在C18柱上以甲酵—水为流动相进行梯度洗脱，在0-10min内将葛根黄酮提取物进行分离，并在250nm检测，采用标准加入法对实际样品中的葛根素含量进行了分析。结果表明：平均回收率为94.8％，RSD为2．4％。
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| 关 键 词： | 反相高效液相色谱法 葛根 黄酮提取物 分离 活性成分 测定 植物药 |
Separation and Determination of Active Components of Pueraria Lobata Isoflavonoid Extract by Reversed Phase High Performance Liquid Chromatography |
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| Li Meiqing ,Sheng Xuan ,Shao Xueguang.Separation and Determination of Active Components of Pueraria Lobata Isoflavonoid Extract by Reversed Phase High Performance Liquid Chromatography[J].Chinese Journal of Analytical Chemistry,2003,31(2):178-180. |
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| Authors: | Li Meiqing Sheng Xuan Shao Xueguang |
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| Institution: | Li Meiqing 1,Sheng Xuan 2,Shao Xueguang *1 1 |
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| Abstract: | A method for the separation and determination of Pueraria lobata isoflavonoid extract by reversed-phase high performance liquid chromatography (RP-HPLC) was proposed. All the components can be separated through a C 18 analytical column with methanol-water as mobile phase and a gradient elution-changing the percentage of the methanol from 15% to 58% in 10 minutes. Puerarin were quantitated by standard addition method at the detection wavelength of 250 nm. The result shows that the average recovery of Puerarin is 94.8%, RSD is 2.4%. |
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| Keywords: | Pueraria lobata isoflavonoid extract reversed phase high performance liquid chromatography |
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